P15080: Flow Culture System
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Planning & Execution

Table of Contents

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Project Summary

Cell culture is the process by which cells are grown outside of their natural environments under controlled conditions. Current culture techniques typically grow cells on two-dimensional plastic surfaces under static conditions. Cells in vivo do not live in these conditions, and thus studying cells in these conditions is not representative of physiological conditions. A flow culture system grows and sustains cultured cells in a dynamic environment by maintaining sterile and standard culture conditions. This tool provides a mechanism for constant fluid exchange that does not interfere with cell composition or device sterility. The device also provides a means to view the current state of the culture using microscopy.

The goal of this project is to design a system to be used in the Advanced Cell Culture class within the Biomedical Engineering Department at RIT that will allow students to analyze endothelial cells and their growth in flowing media. The MSD team is tasked with designing a flow culture system capable of maintaining sterile, standard culture conditions while media flows over the cultured cells. The expected result is a functional prototype that controls fluid flow and shear stresses of the media while maintaining CO2 concentrations, temperature, and humidity of the chamber to promote cell growth. This device should provide an appropriate surface onto which the cells can adhere and grow in desired areas. This culture must be able to be viewed with microscopes available currently in the department. The final product needs to be built out of non-cytotoxic, non-fluorescent materials, use a minimal volume of media, maintain sterility during media change, and be able to be sterilized by an autoclave between uses.

For an updated project description, click on the following link for the Project Readiness Package.

Morgan's Notes

Intellectual Property Considerations

Is your team considering filing for a provisional or non-provisional patent? Does your project's financial support carry any IP requirements? Are there select elements of your project that your customer has required that you keep confidential?

Team Roles

Has the team arrived at consensus on a team leader? Who will handle the other important non-design functions, such as facilitator, purchasing, EDGE help, customer POC, etc?

Team Values and Norms

Created in MSD I, updated in MSD II or as needed. Refer to Team Values and Norms page, as well.

Project Plans & Schedules

MSD I & II: work breakdown structure, Gantt charts, schedule, etc. Does each team member know what he should be working on at any given time in order to move the project forward?

Risk Assessment

Created in MSD I and updated throughout the project as needed. What could possibly go wrong? As the project progresses, are you actively managing these risks and (hopefully) driving them to low likelihood and/or low severity?

Meeting Minutes, Notes, & Actions

MSD I & II. Maintain a record of team/guide meetings. This includes discussion, action items, decisions made, and work assigned.

Peer Reviews

These should be conducted after each 3-week phase, in both MSD I & II. Are you using your peer reviews as an objective tool to improve the efficiency and effectiveness of the team and all its members?

Project Reviews

Preparation, notes and actions, etc. Refer to Project Review page, as well.

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