P16083: Automated Microfluidic Cell Separator
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Project Summary Project Information

Our senior design project is to design and develop a microfluidic channel that will be able to sort cells given user input. Current methods of cell separation use biomarker tagging or centrifugal forces to separate cell types. However, both of these methods are costly and can have major impacts on the viability of the cells. Our device will use electric fields to manipulate and sort the cells without the need for expensive reagents or dangerously high shear forces.

Currently, separating a co-culture of cells is a time and labor intensive process. This device will only require the operator to load the sample and set the target signal for the cells. The semi-automation reduces the need for intensive training and reduces the risk of human error.

This project will be used in a teaching lab environment to solidify the understanding of microfluidic techniques to separate cells and particles using dielectrophoresis.

http://www.mdpi.com/1422-0067/15/10/18281/htm

http://www.mdpi.com/1422-0067/15/10/18281/htm

Project Name
Automated Microfluidic Cell Separator
Project Number
P16083
Start Term
2151
End Term
2155
Faculty Guide
Mr. Harold Paschal, paschal@frontiernet.net
Primary Customer
Dr. Blanca Lapizco-Encinas, bhlbme@rit.edu
Sponsor (financial support)
Biomedical Engineering Department at RIT, Student Innovation Center, Multidisciplinary Senior Design Office
Project Proposal
Project Readiness Package

Team Members

Left to Right: Ryan Kinney, Tyler Lisec, Alexandra LaLonde, Jay Dolas, Vincent Serianni II, Chris Molinari

Left to Right: Ryan Kinney, Tyler Lisec, Alexandra LaLonde, Jay Dolas, Vincent Serianni II, Chris Molinari

Member Major Role Contact
Jay Dolas BME Lead Engineer jrd8174@rit.edu
Alexandra LaLonde BME Microfludics Specialist ael9930@rit.edu
Vincent Serianni II BME Project Manager vcs7133@rit.edu
Tyler Lisec ME Lead Mechanical tjl9229@rit.edu
Ryan Kinney EE Lead Electrical rhk9387@rit.edu
Chris Molinari EE Controls Engineer cxm4025@rit.edu

Table of Contents

MSD I & II MSD I MSD II

Project Management

Project Photos and Videos

Imagine RIT

Planning & Execution

Problem Definition

Systems Design

Subsystem Design

Preliminary Detailed Design

Detailed Design

Build & Test Prep

Subsystem Build & Test

Integrated System Build & Test

Integrated System Build & Test with Customer Demo

Customer Handoff & Final Project Documentation

Acknowledgements

Our team would like to thank our guide, Mr. Harold Paschal, for his time and guidance in this project. We would also like to thank Dr. Blanca Lapizco-Encinas for dedicating her time and expertise in the subject matter to keep the project moving forward.