P16083: Automated Microfluidic Cell Separator
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Integrated System Build & Test

Table of Contents

Team Vision

During this phase the team hoped to get a separation of cells using dielectrophoresis. This was an overly optimistic goal, as microfabrication is sensitive and we needed time to work out the kinks and details.

The team actually did move 1um polystyrene particles using dielectrophoresis in a channel which uses the fabrication method by which we plan to use in the product's end state. This alone was a small success. As we progress with our microfabrication, we should ensure less hurdles (such as seals breaking, fluid leaking, etc.) which should allow us to perform the experiments on cells instead of just particles. This will come with the next phase.

Engineering Requirements

ER4 - Variable Voltage

Currently the voltage is able to reach 24VAC this will be improved once rectifier transformer testing is finished.

ER5 - Variable Frequency

ER 5 - Variable Frequency

ER6 - Use Wall Outlet for Power

Currently every electrical component can be powered by a 120V wall outlet.

ER7 - Accurate Timer

Timer was tested add was accurate for a 1 hour run time. This needs to be tested again on an microprocessor that is running with multiple functions.

ER8 - Emergency Shut off

The button has been tested and works as expected.

ER10 - Maximum Current

The maximum current is currently measuring below 15mA.

ER24 - Number of User Inputs

The user currently will be have have 12 inputs to set before the device will begin. The power switch (1), setting the voltages (2), setting the frequency(2), controlling the motors(2), the start button(1), the emergency stop(1),and the electrode safety switch(1).

Stepper Motor

Circuit of Stepper Motor with Arduino and Controller on a Breadboard

Circuit of Stepper Motor with Arduino and Controller on a Breadboard

Minimum Voltage and Frequency needed to run the Stepper Motor

Minimum Voltage and Frequency needed to run the Stepper Motor

Slides of Pictures and Videos

Stepper Motor Results

Voltage Display

LED Voltage Display Circuit with Arduino on Breadboard at 13V

LED Voltage Display Circuit with Arduino on Breadboard at 13V

Power Supply set at 13V, connected to a voltage divider and Arduino for the LED to display

Power Supply set at 13V, connected to a voltage divider and Arduino for the LED to display

LED Voltage Display Circuit with Arduino on Breadboard at 25V

LED Voltage Display Circuit with Arduino on Breadboard at 25V

Power Supply set at 25V, connected to a voltage divider and Arduino for the LED to display

Power Supply set at 25V, connected to a voltage divider and Arduino for the LED to display

Voltage Display Results

Risk and Problem Tracking

Gantt Chart Update

Gantt Chart Update

Risk Update

Risk Update

Functional Demo Materials

Mechanical

1:15mL Tube Rests 2:Microchannel Support 3:Syringe Pump 4:Electrode Wire Terminal Block 5:Fluid Flow Rate Display 6:Frequency Generator 7:Voltage and Current Display 8:Emergency Stop 9:Voltage Control 10:Start Button 11:Fluid Flow Control 12:Syringe Pump Wire Hole

1:15mL Tube Rests 2:Microchannel Support 3:Syringe Pump 4:Electrode Wire Terminal Block 5:Fluid Flow Rate Display 6:Frequency Generator 7:Voltage and Current Display 8:Emergency Stop 9:Voltage Control 10:Start Button 11:Fluid Flow Control 12:Syringe Pump Wire Hole

Modifications Needed

Biological

Channel Seal

With electrodes spaced 250 um from the channel, some leakage occurred primarily around the perpendicular electrode. Electrode slits were cut with an X-Acto knife.
Leaking around perpendicular electrode with 250 um spacing from channel

Leaking around perpendicular electrode with 250 um spacing from channel

Leaking between inlet channel and perpendicular electrode with 250 um spacing from channel

Leaking between inlet channel and perpendicular electrode with 250 um spacing from channel

With electrodes spaced 350 um from the channel, some leakage occurred around the electrodes. In this case, electrode slits were also cut with an X-Acto knife. Because of the leakage that occurred, a new method of cutting electrode slits needed to be found.

Leaking to the right of the parallel electrode in the channel with 350 um electrode spacing

Leaking to the right of the parallel electrode in the channel with 350 um electrode spacing

Leaking between outlet channel and perpendicular electrode with 350 um spacing from channel. Particles fluoresce green, better showing the leak

Leaking between outlet channel and perpendicular electrode with 350 um spacing from channel. Particles fluoresce green, better showing the leak

Leaking to the right of the parallel electrode in the channel with 350 um electrode spacing. Particles fluoresce green, better showing the leak

Leaking to the right of the parallel electrode in the channel with 350 um electrode spacing. Particles fluoresce green, better showing the leak

1 um Particle eDEP

For this experiment, a microchannel with electrodes spacing 350 um from the channel was used. To cut electrode slits, a 1 mm biopsy punch was used to create 1 mm x 5 mm ovular cuts. The channel seal can be seen in the video below. Some leakage occurs between both electrodes and the outlet channels, however overall the channel seal is much better than in previous trials.

Channel Seal Video

The log of DEP tests can be found below.

2016.03.15 DEP Test Log

With a 5 kHz 10 Vpp sine wave, no DEP could be observed.

5 kHz 10 Vpp sine wave

With a 5 kHz 40 Vpp sine wave, some negative DEP force was seen (particles traveling from the perpendicular to the parallel electrode).

5 kHz 40 Vpp sine wave

With a 10 kHz 40 Vpp sine wave, little to no negative DEP force was seen. However, at this point it appears the channel was damaged from repeated movement of the electrodes. Testing at this set of conditions will be repeated.

10 kHz 40 Vpp sine wave

Plans for next phase


Table of Contents

MSD I & II MSD I MSD II

Project Management

Project Photos and Videos

Imagine RIT

Planning & Execution

Problem Definition

Systems Design

Subsystem Design

Preliminary Detailed Design

Detailed Design

Build & Test Prep

Subsystem Build & Test

Integrated System Build & Test

Integrated System Build & Test with Customer Demo

Customer Handoff & Final Project Documentation