P16083: Automated Microfluidic Cell Separator
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Preliminary Detailed Design

Table of Contents

Team Vision for Preliminary Detailed Design Phase

Our team's goals for this phase were to establish the voltages and frequencies needed to create the separation of cells in the Automated Microfluidic Cell Separator. Our second goal was to successfully complete a feasibility cell separation using materials provided to us before making purchases ourselves.

Although neither of these goals were fully reached, each one had significant progress. In terms of finding the voltages and frequencies, our team has begun extensive research of peer reviewed articles. This will continue into the Detailed Design Phase. Our first experiment was successful in making a dielectrophoretic force on polystyrene particles by using a homemade microfluidic device. This shows proof of concept, and leads our team into the Detailed Design Phase with confidence to improve our process and keep moving forward.

Prototyping, Engineering Analysis, Simulation

PSPICE Simulation of Power Supply

PSPICE Simulation of Power Supply

Tested Concept based on subject matter expert's suggestion

Tested Concept based on subject matter expert's suggestion

Result of bread boarding Ardunio signal generator (1Hz-170Hz)

Result of bread boarding Ardunio signal generator (1Hz-170Hz)

CM factor characterization of yeast and e coli

CM factor characterization of yeast and e coli

CM Chart

CM Chart

Graph showing the frequencies that yeast and e coli respond to this separation technique

Graph showing the frequencies that yeast and e coli respond to this separation technique

Drawings, Schematics, Flow Charts, Simulations

Proposed Device

Device Revision #3

Device Revision #3

Flow Simulation

Flow through the channel was modeled using COMSOL finite elements software.

Shear Stress

Shear stress in a three channel configuration

Shear stress in a three channel configuration

Shear stress in a three channel configuration zoomed on the divergence

Shear stress in a three channel configuration zoomed on the divergence

Shear stress in a three channel configuration zoomed on the channel entrance

Shear stress in a three channel configuration zoomed on the channel entrance


Velocity Profile

Velocity profile in a three channel configuration zoomed on the divergence

Velocity profile in a three channel configuration zoomed on the divergence

Velocity profile in a three channel configuration zoomed on the channel entrance

Velocity profile in a three channel configuration zoomed on the channel entrance

Flow Equations

Flow Equations

Note: The mesh size was the finest COMSOL can produce.

Bill of Material (BOM)

Click here to download the Bill of Materials.

Test Plans

Mask used for Testing

Mask used for Testing

public/Detailed Design Documents/FlatChannel Experiment.PNG

public/Detailed Design Documents/FlatChannel Experiment.PNG

public/Detailed Design Documents/FlatChannel ExperimentResults.PNG

public/Detailed Design Documents/FlatChannel ExperimentResults.PNG

public/Detailed Design Documents/FlatChannel ExperimentIssues.PNG

public/Detailed Design Documents/FlatChannel ExperimentIssues.PNG

The test plan for each engineering requirement can be download from the links below:

The Following Test Plans are not Directly Correlated to Engineering Requirements:

Design and Flowcharts

Design if higher voltages are required

Design if higher voltages are required

Proposed Stepper Motor Controller

Proposed Stepper Motor Controller

Risk Assessment

Design Review Materials

Gantt Chart

Gantt Chart Updated for Phase 4

Gantt Chart Updated for Phase 4

Plans for next phase


MSD I & II MSD I MSD II

Project Management

Project Photos and Videos

Imagine RIT

Planning & Execution

Problem Definition

Systems Design

Subsystem Design

Preliminary Detailed Design

Detailed Design

Build & Test Prep

Subsystem Build & Test

Integrated System Build & Test

Integrated System Build & Test with Customer Demo

Customer Handoff & Final Project Documentation