P16083: Automated Microfluidic Cell Separator
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Subsystem Build & Test

Table of Contents

Team Vision for Subsystem Level Build & Test Phase

Our team planned to work on channel designs, more specifically optimizing the smoothness of the channel as well as confirming electrode placement methods. Additionally, we planned to begin machining and 3D printing parts as well as testing electrical components and starting to integrate them.

Progress was made on all of the above mentioned plans.

Test Results Summary

ER5 - Variable Frequency

ER 5 - Variable Frequency

Stepper Motor

Motor Set-up

Motor Set-up

Minimum Voltage and Frequency

Minimum Voltage and Frequency

Slides of Pictures and Videos

Channel Fabrication

Laser Etching

A full-factorial design of experiments was performed to characterize and model etching settings. Laser power, speed, and DPI were tested, as well as line offset and number of parallel lines being etched. The response variables analyzed were channel depth, width, cross-sectional area, smoothness and shape. The results of the analysis in Minitab of this data is below, with only results for depth, width, and cross-sectional area as these are the only quantitative variables.

Responses to alterations in etching settings

Responses to alterations in etching settings

After the data was analyzed, an optimization was run on the data to yield a cross-sectional area of 0.08 mm^2. This optimized set of parameters was tested to ensure the channel had a smooth cross-sectional profile and acceptable shape.

Settings to optimize etching

Settings to optimize etching

This set of parameters was tested multiple times to ensure repeatability of the channel etching.

Cross-sectional view of etched channel at optimized settings

Cross-sectional view of etched channel at optimized settings

Laser Cutting

A full-factorial design of experiments was performed to characterize and model cutting settings. Laser power, speed, and number of repeated passes were tested. The response variables analyzed were channel depth, width, and shape. The results of the analysis in Minitab of this data is below, with only results for depth, and width as these are the only quantitative variables.

Responses to alterations in cutting settings

Responses to alterations in cutting settings

After the data was analyzed, an optimization was run on the data to yield a depth of 1 mm and width of 0.7 mm. However, using the settings tested in this design, these parameters were not achievable.

Settings to optimize cutting

Settings to optimize cutting

Because the desired results were not achievable, the settings of 30% power, 100% speed, and 5 passes were tested as a best guess and proved to be acceptable.

Channel Seal

Seal of Channel Inlet

Seal of Channel Inlet

Seal of Channel Oulet

Seal of Channel Oulet

MSD 2/test channel1.avi

Risk and Problem Tracking

Gantt Chart

public/Subsystem and Test/GanttChartPhase7.PNG

Mechanical Drawings

Plans for next phase


Table of Contents

MSD I & II MSD I MSD II

Project Management

Project Photos and Videos

Imagine RIT

Planning & Execution

Problem Definition

Systems Design

Subsystem Design

Preliminary Detailed Design

Detailed Design

Build & Test Prep

Subsystem Build & Test

Integrated System Build & Test

Integrated System Build & Test with Customer Demo

Customer Handoff & Final Project Documentation