P16083: Automated Microfluidic Cell Separator
/public/

Subsystem Design

Table of Contents

Team Vision for Subsystem-Level Design Phase

Our team plans to analyze in depth the subsystems of our automated cell separator device. We plan to map the specific components that influence the functions these subsystems perform. We will also consider how these subsystems interact with each other to complete our overall device function.

Our team continued feasibility analysis, focusing more in depth on the subsystems level. This focused on the definition of our subsystems, the interactions of components in those subsystems, and mathematical modeling based on currently chosen components. This phase has put our team in position to begin to experimentally test our basic components while simultaneously preparing a preliminary detailed design over the next 3 weeks.

Subsystem Overview

Subsystem Definition

Subsystem Definition

System Flowchart

System Flowchart

System Flowchart

System Flowchart separated by Control, Fluid Flow, and Power

System Flowchart separated by Control, Fluid Flow, and Power

Feasibility: Prototyping, Analysis, Simulation

Electrical Feasibility

PSPICE simulation of varying the feedback resistance value to achieve variable voltages

PSPICE simulation of varying the feedback resistance value to achieve variable voltages

A electrical timer was built and tested and can be used in the current design

A electrical timer was built and tested and can be used in the current design

Bread boarding attempt to build signal generator was unsucessful due to lack of proper components

Bread boarding attempt to build signal generator was unsucessful due to lack of proper components

Sensitivity Analysis on Device Run Time

Sensitivity analysis was completed comparing critical variables and the impact each have on the run time.

Sensitivity analysis was completed comparing critical variables and the impact each have on the run time.

This analysis was completed using the steps shown here. The final equations found in the previous document were written into a Matlab file, see here, in order to adjust the variables more freely. Using the data from our customer requirements and the properties of the material to be used, we found that the device operation will last approximately 2.7 hours. Using the sensitivity analysis, shown above, reducing the volume of fluid to be run through the device, increasing the channel height, and increasing the channel base will decrease the run time of the device. Increasing the channel height will decrease the run time, however it will have minimal impact on the run time, as seen in the sensitivity analysis. In order to drop this run time to below one hour, we plan to decrease the volume of fluid to be sorted and run multiple channels in parallel.

eDEP Feasibility

public/Photo Gallery/CMCalcs1.JPG

public/Photo Gallery/CMCalcs2.JPG

public/Photo Gallery/CMCalcs3.JPG

Equation and image source

These equations were input into MATLAB, and CM values were calculated over varying frequencies. The codes can be found below.

Yeast

E. coli

Drawings, Schematics, Flow Charts, etc.

Below are technical images from each design group.

System Level

Proposed Device

Device Revision #2

Device Revision #2

Mechanical Sub-Assemblies

Click on the links below to open a PDF of the device and main sub-assemblies.

The link below will direct you to 3D PDFs for each of the assemblies above.

(Note: Files can not be viewed using Google Chrome browser unless saved locally and opened using Adobe Reader.)

Microchannel Designs

1mm Base 3-Outlet Channel

1mm Base 3-Outlet Channel

1mm Base 2-Outlet Channel

1mm Base 2-Outlet Channel

2mm Base 3-Outlet Channel

2mm Base 3-Outlet Channel

2mm Base 2-Outlet Channel

2mm Base 2-Outlet Channel

All units are in microns.

Electrode Geometry Designs

Multi-planar Electrode Geometry

Multi-planar Electrode Geometry

Parallel Electrode Geometry with Planar Ground

Parallel Electrode Geometry with Planar Ground

In both images, red indicates electrodes, white is PDMS, and green is the microchannel. All units are in microns.

Electrical Drawings

Electrical Subsystem Layout

Electrical Subsystem Layout

Electrical Main Power

Electrical Main Power

Electrical Fans/Op Amps

Electrical Fans/Op Amps

Electrical Monitoring

Electrical Monitoring

Biological Extensions

Basic Channel Explanation

Basic Channel Explanation

Ideal Cells
Ideal Cell Characteristics

Ideal Cell Characteristics

Yest and E. coli size comparison

Yest and E. coli size comparison

Alternative cells

Alternative Cell Characteristics

Alternative Cell Characteristics

Bacillus Subtilis

Bacillus Subtilis

Source: www.wikiwand.com/de/Bacillus_subtilis

Bill of Materials (BOM)

The system level bill of materials can be found here.

Note: This bill of materials has not officially been released.

Risk Assessment

Click here for the risk assessment.

Design Review Materials

Create link to design review presentation here.

Project Planning

Gantt Chart: Updated Week 9

Gantt Chart: Updated Week 9

Plans for next phase


MSD I & II MSD I MSD II

Project Management

Project Photos and Videos

Imagine RIT

Planning & Execution

Problem Definition

Systems Design

Subsystem Design

Preliminary Detailed Design

Detailed Design

Build & Test Prep

Subsystem Build & Test

Integrated System Build & Test

Integrated System Build & Test with Customer Demo

Customer Handoff & Final Project Documentation