Customer Handoff & Final Project Documentation
Table of Contents
Team Vision for Final Demo and HandoffMSDII Final Phase Summary
- Our goals for this final phase of our project would be dramatically different than our past phases. With testing completed for the most part, our efforts were to be focused on documenting our results, preparing for ImagineRIT, and handing our project off. In addition to the final reviews with the guide and customers, we will be presenting a two minute summary slide a group of other MSD teams. The purpose of this exercise is to share our accomplishments with our peers who may not be aware of our project and it's successes.
- This phase involved a lot of retrospective review and quantitative analysis. Presenting at ImagineRIT was extremely successful and we generated a lot of interest in our project as well as the rest of the SPEX initiatives. Documenting the project's successes have shown that our team mitigated many of the risks that we originally identified early in the project. We still have yet to mitigate the issues with mixing and suspension but these are good things to highlight as we move to hand off the project. While we fell short on these items, our project was able to prove that we can perform some level of spectroscopy with our device. Getting a significant difference in output voltage between different testing conditions shows that our device functions as planned. Additionally it paves the way for future testing with other proteins. The system is modular and can support other LED's, photodiodes, and band-pass filters specific to other proteins.
Test Results Summary
Protein Denaturation Testing
In order to see if the spectroscopy system could detect a difference between folded and unfolded (denatured) proteins, BSA was chemically denatured with urea and then added to a test well. The preliminary results from April 19, 2016 are shown below:
As can be seen, denatured protein resulted in a higher voltage reading. Follow up tests on May 6, 2016 were performed to confirm these results.
These tests confirmed that denatured proteins register at a higher voltage reading. It was expected that when the protein unfolded, the fluorescent tryptophan would be quenched by the PBS reagent and register as a lower voltage reading. The current theory is that the protein unfolded enough to expose the tryptophan to more light without being too exposed to the reagent.
Satisfaction of Customer Requirements
Most of our customer requirements were met, but looking back some of them were out of the scope of the project. In addition, the customer requirements that were only partially met were due to the inability to complete a proper microchannel mixing subsystem.
Satisfaction of Engineering Requirements
|Requirement Number||Engineering Requirement||Link to Test Plan|
|1||Height of Assay||Height|
|2||Length of Assay||Length|
|3||Width of Assay||Width|
|5||Amount of Protein||Protein|
|6||Volume of Reagent||Reagent|
|8||Light Source||Light Source|
|9||Precision Tolerance of Protein Data||Tolerance|
|10||Protein Applicable for Study||Applicable|
|12||Max Assay Voltage||Max Voltage|
|13||Max Power||Max Power|
|14||Computer Chip Storage||Storage|
|15||Transmit and Download Speed of Data||Speed|
|17||Payload Within Budget||Budget|
Risk and Problem Tracking
The current risks have been driven down to two, which deal with the microfluidic mixing system. These risks have not been driven to 0 because the system was unable to be completed due to bubbling during the mixing process. One future vision for this product is to develop a vacuum sealed channel so that the air bubbles do not develop. Unfortunately, we did not possess the resources to recognize this as a reality at this stage.
Link to live Risks document: Final Risk Documentation
Problem Tracking Documentation
Final Project Documentation
Bill of MaterialsLink to PDF document: Final BOM Documentation
Link to live document: Final BOM Documentation
PaperLink to Final Paper: Final Technical Paper
- Final Poster: P16104 ImagineRIT Poster
- Imagine Speech: Team Elevator Speech
Assembly and Schematics Manuals
- Solenoid Mount Print File: 3D Print File
- Side Panel Water Jet File: Water Jetting File
PDMS Design File: PDMS Layer File
NOTE: This file is a .studio3 file and requires the
corresponding program to open it.
- PDMS Assembly Instructions: Well Assembly
- Electrical Board Order and Assembly Process: PCB Assembly
- Device Assembly: Final Assembly Instructions
User ManualUser Instructions
PresentationLink to Final Presentation: Final Presentation
Final Schedule and Planned Wrap Up
Mallory Rauch's Three Week Plan : Mallory's Goals