P16104: Microfluidic Spectroscopy for Proteins within CubeSats
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Customer Handoff & Final Project Documentation

Table of Contents

Team Vision for Final Demo and Handoff

MSDII Final Phase Summary

Test Results Summary

Protein Denaturation Testing

In order to see if the spectroscopy system could detect a difference between folded and unfolded (denatured) proteins, BSA was chemically denatured with urea and then added to a test well. The preliminary results from April 19, 2016 are shown below:

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As can be seen, denatured protein resulted in a higher voltage reading. Follow up tests on May 6, 2016 were performed to confirm these results.

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These tests confirmed that denatured proteins register at a higher voltage reading. It was expected that when the protein unfolded, the fluorescent tryptophan would be quenched by the PBS reagent and register as a lower voltage reading. The current theory is that the protein unfolded enough to expose the tryptophan to more light without being too exposed to the reagent.

Satisfaction of Customer Requirements

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Most of our customer requirements were met, but looking back some of them were out of the scope of the project. In addition, the customer requirements that were only partially met were due to the inability to complete a proper microchannel mixing subsystem.

Satisfaction of Engineering Requirements

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Requirement Number Engineering Requirement Link to Test Plan
1 Height of Assay Height
2 Length of Assay Length
3 Width of Assay Width
4 Weight Limit Weight
5 Amount of Protein Protein
6 Volume of Reagent Reagent
7 Component Validation Validation
8 Light Source Light Source
9 Precision Tolerance of Protein Data Tolerance
10 Protein Applicable for Study Applicable
11 Max Current Current
12 Max Assay Voltage Max Voltage
13 Max Power Max Power
14 Computer Chip Storage Storage
15 Transmit and Download Speed of Data Speed
16 Temperature Temperature
17 Payload Within Budget Budget

Risk and Problem Tracking

The current risks have been driven down to two, which deal with the microfluidic mixing system. These risks have not been driven to 0 because the system was unable to be completed due to bubbling during the mixing process. One future vision for this product is to develop a vacuum sealed channel so that the air bubbles do not develop. Unfortunately, we did not possess the resources to recognize this as a reality at this stage.

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Link to live Risks document: Final Risk Documentation

Problem Tracking Documentation

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Final Project Documentation

Bill of Materials

Link to PDF document: Final BOM Documentation

Link to live document: Final BOM Documentation

Paper

Link to Final Paper: Final Technical Paper

Imagine Materials

Assembly and Schematics Manuals

User Manual

User Instructions

Presentation

Link to Final Presentation: Final Presentation

Final Schedule and Planned Wrap Up

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Individual Plans

Mallory Rauch's Three Week Plan : Mallory's Goals


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