P16104: Microfluidic Spectroscopy for Proteins within CubeSats
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Integrated System Build & Test

Table of Contents

Team Vision for Integrated System Build & Test Phase

MSDII Third Phase Summary:

Current Phase Gantt Chart

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Test Results Summary

Structural Subsystem

Vibrations - Modal Analysis

Model Analysis: Using the overall mass and stiffness of a structure model analysis is used to find the periods at which the structure will naturally resonate. NASA CubeSat requirements prohibit 1st resonance frequency to be above 100 Hz

Modeling: Assumptions: Specifics regarding internal components unknown. Shapes and sizes were estimated to serve as placeholders CubeSat launched from PicoSatellite Orbital Deployer (P-POD) P-POD is constrained along the rails (sides), but it allowed slight movement in the vertical direction. Constrained in both x and y directions and allowed slight freedom in z.

Results: Mode 1:

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Mode 1: 56.271Hz

Mode 2: 152.1Hz

Mode 3: 156.3Hz

Mode 4: 157.75Hz

Mode 5: 171.79Hz

Mode 1 is significantly lower than 100 Hz. Changes to internal components should not result in drastic changes.

Electrical Subsystem

Snippet of the command line interface for our project

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Accompanying Arduino Code

OpAmp Benchmarking

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Datasheets from Google Drive link in image:

Boards for Manufacture

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LED Board

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Mainboard

Biomedical Subsystem

3-Methylindole Testing Results

3-Methylindole Test Plan

Photodiode Benchmarking

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Datasheets for original photodiode and new photodiode from Google Drive link in image:

Summary of Mixing

Different PDMS layer thicknesses were used as the cover for the microfluidic channel:

As in past iterations, the microchannel was assembled with layers of PDMS sealed with a corona wand. The cover layer was left off until testing time, where the reagent and protein were added to their respective channels, and then the cover was corona treated and applied to the top to complete the microchannel.

When the 100 micrometer cover was subjected to the solenoids, numerous leaks immediately appeared. This cover was deemed too thin for intended usage.

When the 600 micrometer cover was subjected to the solenoids, there was minimal evidence of leaking. However, there was still the issue of bubbling due to the mixing of the reagent with air that was already in the channel. There are two steps that can be taken to avoid this problem:

Due to this issue, the plan for quantification of mixing has not been executed yet.

Preliminary Backup Plans

Green Fluorescent Protein - Backup Plan A

Fluorescent Dye - Backup Plan B

Risk and Problem Tracking

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Link to the live Risks document: Integrated System Build and Test Risks

Problem Tracker

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Plans for next phase

Mallory Rauch's Three Week Plan : Mallory's Goals

Anna Jensen's Three Week Plan : Anna's Goals

James Lewis's Three Week Plan : James's Goals

Darin Berrigan's Three Week Plan : Darin's Goals


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