P18081: Mechanical Bioreactor
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Build & Test Prep

Table of Contents

Team Vision for Build & Test Prep Phase

This phase was largely dedicated to designing the electrical system for the device. The CAD models for the device were finalized so that a prototype model could be 3-D printed and assembled.

Test Plan Summary



TABLE 1:Updated Test Plan List (click link to view test plan outline)

Test Plan # Engineering Requirement Title Purpose
1 2,3,4 Environmental Conditions To ensure that the incubator is maintaining proper temperature, humidity, and CO2 levels.
2 1,5,6,18 Cell Growth/Viability Monitor pH level with media color indicator. Confirm sterile environment with cell proliferation counts and cell attachment.
3 15, 16 Autoclave Testing Determine if selected materials for mechanical strain applicator can withstand autoclave conditions.
4 3 Material Testing Tensile Testing using ASTM standard D638.
5 5,9,13,14 Ease of Use Assembly/Disassembly, Protocol, Intuitive Software, etc.
6 10,14,19,20 Chamber Design Test Ensure chamber growth surface area is sufficient for cell growth and viewable with microscope. Chamber height compatible with microscope.
7 9 Software and Device Motion Testing Verify that software performance is acceptable and it is producing desired outputs.
8 22 Cost Determine whether project development costs are fully funded by allocated MSD funds.
9 23,24 Longevity Testing Determine whether the device will withstand multiple uses (equal to five years with ten uses per year).
10 11 Heat Cure Testing Determine whether different heat cure conditions affect the material properties of PDMS.
11 11 Cellular Response to Strain Determine the effects of different strain % and frequency of strain on the cell culture formation.

Risk and Problem Tracking

See updated Risk Management Chart here.

Bill of Material (BOM)

Figure 1: Bill of Material: Phase I Update. Items highlighted in green have been ordered. Boxed items were ordered during this phase.

Figure 1: Bill of Material: Phase I Update. Items highlighted in green have been ordered. Boxed items were ordered during this phase.

Design Review Materials

TEAM MYSCLE'S Build & Test Prep Review Presentation can be found here.

Plans for next phase

During the next phase, the team will build a working prototype of the mechanical bioreactor.
Figure 2: MSD II Phase II Project Plan

Figure 2: MSD II Phase II Project Plan

Figure 3: MSD II Phase III&IV Project Plan

Figure 3: MSD II Phase III&IV Project Plan


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