Academic cell culture is primarily performed on two-dimensional plastic surfaces and kept under static conditions. Static, 2-D cell cultures are not representative of the cells’ environment in-vivo since they depend on chemical conditions only. The main goal for this project is to develop a culture chamber that allows in-vitro research to more closely resemble in-vivo conditions by providing chemical and mechanical stimulation. The culture chamber should maintain sterile, standard culture conditions (5% CO2, 37 degrees Celsuis), while applying tensile strain on cultured cells. The culture chamber should fit generic, EVOS, and Leica microscopes for viewing cells, and it should be safe and intuitive for use with an undergraduate student population.

Project Name

Mechanical Bioreactor

Project Number

P18081

Project Family

Biomedical Systems and Technologies

Start Term

2171

End Term

2175

Faculty Guide

Michael Zona

Primary Customer

Jennifer Bailey

Sponsor (financial support)

RIT Biomedical Engineering Department

Planning & Execution

Project Photos and Videos

IMAGINE LINK

Problem Definition

Systems Design

Preliminary Detailed Design

Detailed Design

Build & Test Prep

Subsystem Build & Test

Integrated System Build & Test

Customer Handoff & Final Project Documentation

PRP

Requirements

Risk Management

Problem Tracking

MSD I Meeting Notes

MSD II Meeting Notes

Use Cases

Benchmarking

Functional Decomposition

Morphological Chart & System Alternatives

Pugh Concept Selection

BOM

Mechanical Drawings

Arduino Code

Full Bioreactor Protocol

Prototyping

Test Plans

Simulations

Test Results

Technical Paper

Poster