P18081: Mechanical Bioreactor
/public/

Home

Project Summary Project Information

Academic cell culture is primarily performed on two-dimensional plastic surfaces and kept under static conditions. Static, 2-D cell cultures are not representative of the cells’ environment in-vivo since they depend on chemical conditions only. The main goal for this project is to develop a culture chamber that allows in-vitro research to more closely resemble in-vivo conditions by providing chemical and mechanical stimulation. The culture chamber should maintain sterile, standard culture conditions (5% CO2, 37 degrees Celsuis), while applying tensile strain on cultured cells. The culture chamber should fit generic, EVOS, and Leica microscopes for viewing cells, and it should be safe and intuitive for use with an undergraduate student population.

Put Sponsor logo(s) here
Project Name
Mechanical Bioreactor
Project Number
P18081
Project Family
Biomedical Systems and Technologies
Start Term
2171
End Term
2175
Faculty Guide
Michael Zona
Primary Customer
Jennifer Bailey
Sponsor (financial support)
RIT Biomedical Engineering Department

VISTING FROM IMAGINE? CHECK OUT OUR IMAGINE LINK HERE.

Team Members

TEAM MYSCLE (left to right): Emily Wood, Natalie Nold, Simran Singh, Emily Adams, Amanda Castagnino

TEAM MYSCLE (left to right): Emily Wood, Natalie Nold, Simran Singh, Emily Adams, Amanda Castagnino

Member Role Contact
Amanda Castagnino Biomedical Engineer, EDGE Facilitator axc9355
Natalie Nold Biomedical Engineer, Project Manager nmn5966
Emily Wood Mechanical Engineer, Software Expert, Treasurer enw4374
Emily Adams Mechanical Engineer, Team Facilitator exa4926
Simran Singh Biomedical Engineer, Cell Culture Expert, Notetaker sxs2843

Work Breakdown: By Phase

MSD I & II MSD I MSD II

Planning & Execution

Project Photos and Videos

IMAGINE LINK

Problem Definition

Systems Design

Preliminary Detailed Design

Detailed Design

Build & Test Prep

Subsystem Build & Test

Integrated System Build & Test

Customer Handoff & Final Project Documentation

Work Breakdown: By Topic

Project Management Design Tools Design Documentation Implementation Validation Presentation & Dissemination

PRP

Requirements

Risk Management

Problem Tracking

MSD I Meeting Notes

MSD II Meeting Notes

Use Cases

Benchmarking

Functional Decomposition

Morphological Chart & System Alternatives

Pugh Concept Selection

BOM

Mechanical Drawings

Arduino Code

Full Bioreactor Protocol

Prototyping

Test Plans

Simulations

Test Results

Technical Paper

Poster

Acknowledgements

Special thanks for Michael Zona, Jennifer Bailey, and the RIT Biomedical Engineering Department.

Thanks to ASTM International for additional funding.

Additional thanks for Michelle Horan, Dr. Blanca Lapizco-Encinas & Lab, Gary Hodenius, Dr. Vinay Abhyankar, Dr. Steven Day, Professor William Humphrey, Daniel Zinobile, Dr. Thomas Smith, the RIT Machine Shop, and the RIT Construct.