P18081: Mechanical Bioreactor
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Problem Definition

Table of Contents

Team Vision for Problem Definition Phase

During the Problem Definition phase, TEAM MYSCLE established customer needs and associated engineering requirements for the mechanical bioreactor. To do this, the project customer was interviewed and use cases were created to identify and prioritize features of the bioreactor. This will allow the team to purposefully design the system in the next project phase.

Project Summary

Academic cell culture is primarily performed on two-dimensional plastic surfaces and kept under static conditions. Static, 2-D cell cultures are not representative of the cells’ environment in-vivo since they depend on chemical conditions only. The main goal of this project is to develop a culture chamber that allows in-vitro research to more closely resemble in-vivo conditions by providing chemical and mechanical stimulation. The culture chamber should maintain sterile, standard culture conditions (5% CO2, 37 degrees Celsius), while applying tensile strain on cultured cells. The culture chamber should fit generic, EVOS, and Leica microscopes for viewing cells, and it should be safe and intuitive for use with an undergraduate student population.

Use Cases

Figure 1: Use Case Scenario (Experiment Initiation/Setup)

Figure 1: Use Case Scenario (Experiment Initiation/Setup)

Figure 2: Use Case Scenario (Experiment Execution)

Figure 2: Use Case Scenario (Experiment Execution)

Figure 3: Use Case Scenario (Experiment Analysis/Clean-Up)

Figure 3: Use Case Scenario (Experiment Analysis/Clean-Up)

Project Goals and Key Deliverables

TEAM MYSCLE will work to create a bioreactor that

TEAM MYSCLE will provide the following deliverables:

Constraints

Our device must be appropriate for an undergraduate student lab. This requires a cell culture with biohazard level 1, device dimensions that fit in half of a standard incubator, reasonable assembly and disassembly time for a three-hour lab, and the ability for students to monitor the cells during a three-week experiment.

A budget of $700 is provided for the development of our device.

Customer Requirements (Needs)

Figure 4: Customer Requirements ( 3 = Very Important, 2 = Important, 1 = Somewhat Important )

Figure 4: Customer Requirements ( 3 = Very Important, 2 = Important, 1 = Somewhat Important )

Engineering Requirements (Metrics & Specifications)

Figure 5: Engineering Requirements

Figure 5: Engineering Requirements

House of Quality

Figure 6: House of Quality ( 3 = Strong Correlation, 3 = Medium Correlation, 9 = Weak Correlation )

Figure 6: House of Quality ( 3 = Strong Correlation, 3 = Medium Correlation, 9 = Weak Correlation )

Design Review Materials

TEAM MYSCLE'S Project Plan is outlined in Problem Definition Design Review Presentation.

Plans for next phase

TEAM MYSCLE'S Project Plan is outlined in the MSDI Gantt Chart.

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