P18081: Mechanical Bioreactor/public/
Table of Contents
Team Vision for Problem Definition PhaseDuring the Problem Definition phase, TEAM MYSCLE established customer needs and associated engineering requirements for the mechanical bioreactor. To do this, the project customer was interviewed and use cases were created to identify and prioritize features of the bioreactor. This will allow the team to purposefully design the system in the next project phase.
Project SummaryAcademic cell culture is primarily performed on two-dimensional plastic surfaces and kept under static conditions. Static, 2-D cell cultures are not representative of the cells’ environment in-vivo since they depend on chemical conditions only. The main goal of this project is to develop a culture chamber that allows in-vitro research to more closely resemble in-vivo conditions by providing chemical and mechanical stimulation. The culture chamber should maintain sterile, standard culture conditions (5% CO2, 37 degrees Celsius), while applying tensile strain on cultured cells. The culture chamber should fit generic, EVOS, and Leica microscopes for viewing cells, and it should be safe and intuitive for use with an undergraduate student population.
Project Goals and Key DeliverablesTEAM MYSCLE will work to create a bioreactor that
- Sustains cell life for a three-week experiment.
- Exerts mechanical tension on cells to simulate in-vivo conditions.
- Allows cell viewing during experiment and viewing using standard lab microscopes.
- Provides at least two culture conditions for students to compare.
- Is safe and intuitive for undergraduate student use.
- Allows collection of cells for analysis after experiment completion (optional).
TEAM MYSCLE will provide the following deliverables:
- Mechanical bioreactor device
- LabView software interface for operating device
- Engineering documentation completed during MSD
- Protocol for using the device
- Sample experimental protocol for Advanced Cell Culture lab
ConstraintsOur device must be appropriate for an undergraduate student lab. This requires a cell culture with biohazard level 1, device dimensions that fit in half of a standard incubator, reasonable assembly and disassembly time for a three-hour lab, and the ability for students to monitor the cells during a three-week experiment.
A budget of $700 is provided for the development of our device.