P18081: Mechanical Bioreactor
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Prototyping

Prototyping

CELL CHAMBER MOLD:
The original mold design was 3D printed and tested. Attempting to mold a PDMS chamber (see Figure 8 for PDMS molded chamber), design flaws were identified and discussed for the second iteration of the mold design. A few of the major problems encountered during the molding process were no visibility of the PDMS solution within the mold and failure to open mold post curing. The mold was redesigned to include viewing "windows" and eliminate the lip on the lid. CAD models for both the original and updated mold designs are found below. Figure 5 illustrates both of the 3D mold prints in a side-by-side comparison.
Figure 1: Original Mold (Top)

Figure 1: Original Mold (Top)

Figure 2: Original Mold (Bottom)

Figure 2: Original Mold (Bottom)

Figure 3: Mold 2.0 (Top)

Figure 3: Mold 2.0 (Top)

Figure 4: Mold 2.0 (Bottom)

Figure 4: Mold 2.0 (Bottom)

Figure 5: 3D Printed Molds- Original Mold (Left) & Updated Mold (Right)

Figure 5: 3D Printed Molds- Original Mold (Left) & Updated Mold (Right)

Figure 6: Molded PDMS (from Original Design)

Figure 6: Molded PDMS (from Original Design)


ACTUATING SYSTEM ASSEMBLY:
The CAD model below illustrates the full assembly of the cell straining device. The device utilizes a hole and peg cell chamber, along with a purchasable actuator that mechanically strains the cell chamber.

Figure 7: Preliminary Actuator Design: Full Assembly

Figure 7: Preliminary Actuator Design: Full Assembly