P18082: Electrical Bioreactor
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Build & Test Prep

Table of Contents

Team Vision for Build & Test Prep Phase

Team roles and responsibilities are reorganized following the completion of Senior Design I. The Team Manager role is no longer divided between two team members with now one Team Manager.

Member Role
Luc Belikis Mechanical Design Lead
Dillon Flood Risk Management Engineer, Procurement, Secretary
Jonathan Girard Electrical Design Lead
Emily Kimber Team Manager, Edge Expert
Shena Marshall Biomedical Engineer Expert, Communication Director

Test Plan Summary

P18082 Test Plan List
View Test Plan Purpose Start/Completion Dates Conclusion Comments
[Test Plan 1] Test Plan 1: Electrode and Microcontroller Durability Test and Voltage Test To determine whether the pH of media will change due to electrode material/voltage and if the system applies a measurable voltage across the cell culture O Over the course of 7 days, the media reacted the least negativity with carbon electrodes. The measured voltage was steady. A longer test of 21 would be preferred but time and
[Test Plan 2] Test Plan 2: Accessibility Test To determine whether the electrical bioreactor assembly is usable for holding the culture system X -- --
[Test Plan 3] Test Plan 3: Assembly Test and Control System Ease of Use To determine how long it takes to assemble the reactor from gathering materials to placement in the incubator; To determine intuitiveness from LabVIEW controls and robustness of the LabVIEW/Arduino interface X -- --
[Test Plan 4] Test Plan 4: Sterilization/Cleaning Test To identify the time required to (deep) clean the system and issues resulting due to system material properties and determine if Ethanol sterilization is enough to fully sterilize the system X -- --
[Test Plan 5] Test Plan 5: System Durability Test To ensure that system will be able to withstand rough handling of the system X -- --
[Test Plan 6] Test Plan 6: Biocompatibility System To ensure the electrode material selected is not toxic to the cells X -- --
[Test Plan 7] Test Plan 7: Electrical Requirement Verification Test To determine that the electrical requirements are met X -- --
[Test Plan 8] Test Plan 8: Full-Run Electrical Bioreactor Test To determine whether the cells will live and be viable throughout the duration of the system X -- --

Risk and Problem Tracking

P18082 Problem Tracking Phase I

P18082 Problem Tracking Phase I

Drawings, Schematics, Flow Charts, Simulations

Mechanical

P18082 Electrical Bioreactor Design 3.25.18

P18082 Electrical Bioreactor Design 3.25.18

P18082 Electrical Bioreactor 3.25.18 Expanded View Base Plate

P18082 Electrical Bioreactor 3.25.18 Expanded View Base Plate

P18082 Electrical Bioreactor 3.25.18 Expanded View Arduino Housing

P18082 Electrical Bioreactor 3.25.18 Expanded View Arduino Housing

P18082 Electrical Bioreactor 3.25.18 Expanded View Back Walll

P18082 Electrical Bioreactor 3.25.18 Expanded View Back Walll

Electrical

P18082 LabVIEW Interface and Codel

P18082 LabVIEW Interface and Codel

Plans for next phase

The plans for next phase are to complete the below listed items.

1. Integrate LabVIEW with Arduino 2. Hardware/Software Integration Test 3. Rerun Test Plan 1: pH Test at 1V 4. Aseptic Technique Review 5. Build Bioreactor: Machine Shop 6. Assemble Bioreactor: Machine Bioreactor Assembly Test

Phase I: Build & Prep Presentation Documents

Link to P18082 MSD I Phase I Presentation


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