P18082: Electrical Bioreactor
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Detailed Design

Table of Contents

Team Vision for Detailed Design Phase

Mechanical:

-The bioreactor drawings need to be finalized and ready to be used in January: spring mechanism needs to be implemented and updated design needs to account for new wiring schematic

Electrical

- Additional testing needs to be conducted to finalize for a selected electrode material
- Circuit Diagrams/Schematics need to be finalized: a Pulse-Width-Modulation will be used for inducing a cyclic voltage on the media

Biological

-BME team members need to assist with electrode testing: measure effects of electrode to media reactions based on applied voltages
-Ensure all necessary testing material and equipment is ordered and ready for test plans at start of MSDII

Managerial

-Create clear vision and schedule for December through the end of February
-Ensure all necessary tasks are met by each team member
-Prioritise risks, making sure to tactical the highest risks first

Logistical

-Update BOM at end of each meeting to ensure team is accounting for all necessary items
-Update Risk Assessment at end of each meeting and test plan to highlight items to be addressed

Progress Report

P180821 Progess Report

P180821 Progess Report

P180821 Progess Report Continued

P180821 Progess Report Continued

Prototyping, Engineering Analysis, Simulation

Electrode Research

P18082 Electrode Research

P18082 Electrode Research

Frequency Research

Research shows that when a voltage range between 1V and 5V is applied to a petri dish with an electrode configuration similar to our design, the frequency is not applied over a maximum value of 10 Hz. A study was performed on mouse cardiac muscles where frequency dependence of contraction and [Ca2+]i transients was studied in mice ventricular myocytes. To model the heart's function outside of the hosts body, the frequency values of 1, 2, and 4 Hz at 5ms square wave pulses were used to mimic internal conditions for depolarization steps of -70mV to +20mV and repolarization of -50mV.

Additional research produced the below information on carbon electrodes and the respective frequencies used for each. The research suggests we move forward with frequency values of 1, 2, 3, and 4 Hz to determine the best fit for the cell line used in this project.

Cell Line Electrode Length (cm) Electrode Spacing (cm) Voltage Applied (V) Frequency Applied (Hz)
Neonatal Rat Ventricular Myocytes 4 1 3 1,3,5
Engelbreth-Holm-Swarm (EHS) Mouse Sarcoma 4 1 5 1

Electrode Testing

Physical and Chemical Media Observations Continued

Physical and Chemical Media Observations Continued

Physical and Chemical Media Observations on Carbon

Physical and Chemical Media Observations on Carbon

P180821 Test Failures

P180821 Test Failures

Drawings, Schematics, Flow Charts, Simulations

Circuit Schematic

P18082 Circuit Diagram

P18082 Circuit Diagram

P18082 Initialize Variables

P18082 Initialize Variables

P18082 Void Function

P18082 Void Function

P18082 Calculations

P18082 Calculations

P18082 Serial Return

P18082 Serial Return

Bioreactor Schematic

P18082 Exploeded View

P18082 Exploeded View

P18082 Back View

P18082 Back View

P18082 Front View

P18082 Front View

P18082 Front Section View

P18082 Front Section View

P18082 Side View

P18082 Side View

Bill of Material (BOM)

Link to live P18082 Bill of Materials.xls

Test Plans

P180821 Test Plans

P180821 Test Plans

Link to live P18082 Test Plans

Risk Assessment

Link to live P18082 Detailed Design Risk Assessment

Plans for next phase

Link to live P18082 MSDII Schedule


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