P18082: Electrical Bioreactor
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Project Summary Project Information

Project Summary

Cell culturing is commonly performed on two-dimensional plastic surfaces in carefully monitored environments. Several factors contribute to the environment, namely sterility, temperature, humidity, CO2 levels, and media pH levels. However, these conditions are not fully representative of cell growth in vivo (i.e. in living tissue). As such, culturing cells with varied stimuli is often desired in order to emulate in vivo conditions.

The goal of this project is to develop a modified culturing chamber which uses electrical voltage to stimulate the cells. This device will be used by the Biomedical Engineering Department at Rochester Institute of Technology to study precardiomyocytes in its Advanced Cell Culture class taught by Doctor Bailey. The bioreactor will safely keep the culture at the required conditions of sterility, 5% CO_2, and 37 C with a relatively neutral pH for at least 3 weeks, while applying a static or cyclic voltage. The formfactor must fit generic, EVOS, Leica microscopes and utilize LabVIEW and MyDAQ software programs for controlling and monitoring chamber conditions.

Project Name
Electrical Bioreactor
Project Number
P18082
Project Family
Biomedical Systems and Technologies
Start Term
2171
End Term
2175
Faculty Guide
Michael Zona
contact info: mfzddm@rit.edu
Primary Customer
Dr. Jennifer Bailey
Sponsor
Dr. Jennifer Bailey
RIT Biomedical Engineering Department

Team Members

FIG1. Electrical Bioreactor Team

FIG1. Electrical Bioreactor Team

Member Role Contact
Luc Belikis Mechanical Design Lead lxb6779@rit.edu
Dillon Flood Risk Management Engineer, Procurement, Secretary dxf5395@rit.edu
Jonathan Girard Electrical Design Lead jxg2361@rit.edu
Emily Kimber Team Manager, Edge Expert eek8377@rit.edu
Shena Marshall Biomedical Engineer Expert, Communication Director sxm9610@rit.edu

Work Breakdown: By Phase

MSD I & II MSD I MSD II

Planning & Execution

Project Photos and Videos

Imagine RIT

Gate Reviews

Problem Definition

Systems Design

Preliminary Detailed Design

Detailed Design

Build & Test Prep

Subsystem Build & Test

Integrated System Build & Test

Customer Handoff & Final Project Documentation (Verification & Validation)

Work Breakdown: By Topic

Project Management Design Tools Design Documentation Implementation Validation Presentation & Dissemination

PRP

Requirements

Schedule

Cost

Risk Management

Problem Management

Communication & Minutes

Use Cases

Benchmarking

Functional Decomposition

Morphological Chart

Pugh Concept Selection

BOM

Mechanical Drawings

Electrical Schematics

Manuals

Prototyping

Test Plans

Test Results

Design Review Documents

Technical Paper

Poster

Acknowledgements

The team would like to thank Dr. Bailey and the RIT Biomedical Engineering Department for selecting us for this project to develop an electrical bioreactor for the BIME 470 class. We would also like to personally thank Michael Zona for being our Senior Design guide and mentor.